Journal: Applied Sciences

Article Title: Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells

doi: 10.3390/app10228289

Figure Lengend Snippet: Figure 3. Confocal microscopy analysis of the distribution pattern of pan-cadherin (A,B), beta1 integrin (C,D), and vimentin (E,F) in TCam-2 seminoma cells cultured for 24 h at 1 g (CTR: A,C,E) and in simulated microgravity (RPM: B,D,F). BAR: 60 µm.

Article Snippet: MA1-91128, Thermo Fisher Scientific, Monza, Italy), or a rabbit polyclonal anti beta1 integrin (1:500 dilution, clone M106 cod. sc-8978, Santa Cruz Biotechnology), or a mouse monoclonal anti vimentin (1:500 dilution, clone V9 cod.

Techniques: Confocal Microscopy, Cell Culture

Journal: Applied Sciences

Article Title: Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells

doi: 10.3390/app10228289

Figure Lengend Snippet: Figure 5. Confocal microscopy analysis of double-immunofluorescence staining of beta1 integrin (green signal) and vimentin (red signal) in TCam-2 seminoma cells exposed to simulated microgravity for 24 h. In the bottom panel, the merging picture is reported showing that Vimentin does not co-localize with beta1 integrin (yellow signal).

Article Snippet: MA1-91128, Thermo Fisher Scientific, Monza, Italy), or a rabbit polyclonal anti beta1 integrin (1:500 dilution, clone M106 cod. sc-8978, Santa Cruz Biotechnology), or a mouse monoclonal anti vimentin (1:500 dilution, clone V9 cod.

Techniques: Confocal Microscopy, Staining

Journal: Applied Sciences

Article Title: Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells

doi: 10.3390/app10228289

Figure Lengend Snippet: Figure 6. Representative immunoblots of beta1 integrin, pan-cadherin, and vimentin expression levels (A) and the corresponding densitometric analyses (B). These last ones were performed on bands at the corresponding molecular weight of beta1 integrin, pan-cadherin, vimentin, and GAPDH. In addition, they were plotted as a ratio between RPM and CTR, each calculated by the ratio between OD × mm2 of each band and OD × mm2 of the respective band in the immunoblot of the GAPDH, considered as a loading control. In the densitometric analyses, data are means ± SEM from three independent experiments. * p < 0.05.

Article Snippet: MA1-91128, Thermo Fisher Scientific, Monza, Italy), or a rabbit polyclonal anti beta1 integrin (1:500 dilution, clone M106 cod. sc-8978, Santa Cruz Biotechnology), or a mouse monoclonal anti vimentin (1:500 dilution, clone V9 cod.

Techniques: Western Blot, Expressing, Molecular Weight, Control

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: Primer table for Real-Time Quantitative Reverse-Transcriptase PCR (qRT-PCR). Primers were synthesized by MWG Eurofins (Ebersberg, Germany).

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Synthesized

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: Primary antibodies used in Western Blot analysis, Immunofluorescence and Proximity Ligation Assays

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Western Blot, Immunofluorescence, Ligation

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: FKBP10 deficiency alters the expression of molecules implicated in adhesion and migration. a, c, e, g, i Western Blot analysis of phLF treated with scrambled siRNA as control (sc) or FKBP10 siRNA (kd) and 2- phosphoascorbate (0.1 mM) in absence and presence of TGF-β1 (2 ng/mL) for 48h. Densitometric analysis and representative blots show the effect of FKBP10 kd on the expression of talin-1 (TLN1) ( a ), calpain-4 (CAPNS1) ( c ), integrin β1 (ITGB1) ( e ), caveolin-1 (CAV1) ( g ) and coronin 1C (CORO1C) ( i ) relative to β-actin as loading control (ACTB). b, d, f, h, j Quantitative reverse transcriptase-polymerase chain reaction analysis of phLF treated with scrambled siRNA as control (sc) or FKBP10 siRNA (kd) and 2- phosphoascorbate (0.1 mM) in absence and presence of TGF-β1 (2 ng/mL) for 48h. Transcript levels are presented as -ΔCt values showing the effect of siRNA mediated kd of FKBP10 on talin-1 (TLN1) ( b ), calpain-4 (CAPNS1) ( d ), integrin β1 (ITGB1) ( f ), caveolin-1 (CAV1) ( h ) and coronin 1C (CORO1C) ( j ). DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8) was used as endogenous control. All data is based on eight (protein) or seven (mRNA) independent experiments. Statistical significance between control and FKBP10 kd is indicated by horizontal brackets and asterisks

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Expressing, Migration, Western Blot, Polymerase Chain Reaction